Volume 24, Issue 4 (July - August 2025)
Payesh 2025, 24(4): 563-573 |
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Ethics code: IR.MUMS.MEDICAL.REC.1400.436
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Hashemian A M, Raoufinia R, RashidTorabi Z, Hajali V, Ghavidel Yazdi S, Saburi E. The relationship between rs4986790 polymorphism in TLR4 gene and its association with severity of the COVID-19. Payesh 2025; 24 (4) :563-573
URL: http://payeshjournal.ir/article-1-2512-en.html
URL: http://payeshjournal.ir/article-1-2512-en.html
Amir Masoud Hashemian1 
, Ramin Raoufinia2 , Zahra RashidTorabi3 , Vahid Hajali4 , Somayeh Ghavidel Yazdi5 , Ehsan Saburi *6
, Ramin Raoufinia2 , Zahra RashidTorabi3 , Vahid Hajali4 , Somayeh Ghavidel Yazdi5 , Ehsan Saburi *6
1- Department of emergency medicine, faculty of medicine, Mashhad University of Medical Sciences, Mashhad, Iran
2- Noncommunicable Diseases Research Center, Neyshabur University of Medical Sciences, Neyshabur, Iran
3- Department of Medical Genetics and Molecular Medicine, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran
4- Department of Neuroscience, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran
5- Samen Health center, Mashhad University of Medical Sciences, Mashhad, Iran
6- Medical Genetics Research Center, Mashhad University of Medical Sciences, Mashhad, Iran
2- Noncommunicable Diseases Research Center, Neyshabur University of Medical Sciences, Neyshabur, Iran
3- Department of Medical Genetics and Molecular Medicine, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran
4- Department of Neuroscience, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran
5- Samen Health center, Mashhad University of Medical Sciences, Mashhad, Iran
6- Medical Genetics Research Center, Mashhad University of Medical Sciences, Mashhad, Iran
Abstract: (268 Views)
Objective(s): Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has led to severe respiratory illnesses and fatalities in both young and elderly individuals without underlying diseases; however, similar groups may also include individuals who are asymptomatic or exhibit mild symptoms. Various studies have indicated that host genetics play a significant role in the severity of diseases caused by SARS-CoV-1 and MERS-CoV, which share substantial similarities with SARS-CoV-2. One of the crucial host genetic factors involved in identifying patterns of disease-causing agents and activating the innate immune system's Toll-like receptors (TLRs) is TLR4, which shows the strongest association with surface markers of SARS-CoV-2. This study was designed to investigate the common polymorphism of the TLR4 gene (Asp299Gly) to examine the relationship between disease severity and the presence of this polymorphism.
Methods: A total of 230 COVID-19 patients, comprising 154 patients with mild COVID-19 and 76 patients with severe COVID-19, were randomly selected and matched for age and gender from individuals referred to COVID-19 healthcare centers. Peripheral blood samples of 5 mL were obtained from the patients, and DNA was extracted for the analysis of the Asp299Gly-TLR4 polymorphism using the Tetra-ARMS PCR technique.
Results: The study revealed that 67% of the patients belonged to the mild COVID-19 group, while 33% were in the severe COVID-19 group. The analysis showed no significant correlation between disease severity and the Asp299Gly-TLR4 polymorphism. The frequencies of alleles and genotypes did not exhibit a significant relationship with disease severity in either the mild or severe COVID-19 groups.
Conclusion: The findings suggest that there is no significant association between the severity of COVID-19 and the rs4986790 polymorphism in the TLR4 gene. However, further investigation with larger sample sizes is warranted to confirm the effects of different TLR4 polymorphisms on the severity of COVID-19.
Methods: A total of 230 COVID-19 patients, comprising 154 patients with mild COVID-19 and 76 patients with severe COVID-19, were randomly selected and matched for age and gender from individuals referred to COVID-19 healthcare centers. Peripheral blood samples of 5 mL were obtained from the patients, and DNA was extracted for the analysis of the Asp299Gly-TLR4 polymorphism using the Tetra-ARMS PCR technique.
Results: The study revealed that 67% of the patients belonged to the mild COVID-19 group, while 33% were in the severe COVID-19 group. The analysis showed no significant correlation between disease severity and the Asp299Gly-TLR4 polymorphism. The frequencies of alleles and genotypes did not exhibit a significant relationship with disease severity in either the mild or severe COVID-19 groups.
Conclusion: The findings suggest that there is no significant association between the severity of COVID-19 and the rs4986790 polymorphism in the TLR4 gene. However, further investigation with larger sample sizes is warranted to confirm the effects of different TLR4 polymorphisms on the severity of COVID-19.
type of study: Case-control |
Subject:
Social Determinants of Health
Received: 2024/04/25 | Accepted: 2025/06/28 | ePublished ahead of print: 2025/09/9 | Published: 2025/09/9
Received: 2024/04/25 | Accepted: 2025/06/28 | ePublished ahead of print: 2025/09/9 | Published: 2025/09/9
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